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In vivo Bredfelts- og to-foton calciumbilleddannelse fra en mus ved hjælp af et stort kranievindue
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August 04, 2022
DOI:
1Department of Neurophysiology, Faculty of Medicine,University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine,University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine,The University of Tokyo
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Den nuværende protokol beskriver at lave et stort (6 x 3 mm2) kranievindue ved hjælp af madindpakning, gennemsigtig silikone og dækglas. Dette kraniale vindue tillader in vivo wide-field og to-foton calcium billeddannelse eksperimenter i samme mus.
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Manita, S., Shigetomi, E., Bito, H., Koizumi, S., Kitamura, K. In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window. J. Vis. Exp. (186), e64224, doi:10.3791/64224 (2022).
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