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发育生物学
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In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development
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发育生物学
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JoVE 杂志 发育生物学
In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

DOI:
10.3791/64172-v

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10:09 min

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June 16, 2022

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, , , ,
1National Centre for Biological Sciences,Tata Institute of Fundamental Research, 2The University of Trans-Disciplinary Health Sciences and Technology

Chapters

  • 00:05Introduction
  • 00:55Egg Handling and Windowing
  • 01:46Microinjecting Plasmids
  • 03:03Electroporation
  • 04:01Basilar Papilla Dissection
  • 06:04Culture of Basilar Papilla Explants
  • 07:41Results: Molecular Mechanisms Underlying Avian Inner Ear Development and Regeneration
  • 09:15Conclusion

Summary

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The chick is a cost-effective, accessible, and widely available model organism for a variety of studies. Here, a series of protocols is detailed to understand the molecular mechanisms underlying avian inner ear development and regeneration.

Tags

Avian Inner EarHair Cell RegenerationChick EmbryoOtotoxicityGene KnockoutOtic VesicleElectroporationT7 Endonuclease Assay

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