This content is Free Access.
从成年非洲绿松石鳉鱼的脑组织中温和分离细胞核，这是一种自然短暂的衰老研究模型
1,756 Views
•
09:28 min
•
August 09, 2022
DOI:
1Leonard Davis School of Gerontology,University of Southern California, 2Molecular and Computational Biology Department,USC Dornsife College of Letters, Arts and Sciences, 3Biochemistry and Molecular Medicine Department,USC Keck School of Medicine, 4Epigenetics and Gene Regulation,USC Norris Comprehensive Cancer Center, 5USC Stem Cell Initiative
Summary
Automatically generated
在这里，我们提出了一种从短寿命脊椎动物模型 Nothobranchius furzeri 的大脑中分离细胞核的方案，用于下游应用，例如单核RNA测序或转座酶可接近染色质测序（ATAC-seq）的单核测定。
Read Article
Cite this Article
Teefy, B. B., Adler, A., Bhala, R., Benayoun, B. A. Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research. J. Vis. Exp. (186), e64165, doi:10.3791/64165 (2022).
Copy