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Deep Fluorescence Observation in Rice Shoots via Clearing Technology
Chapters
- 00:04Introduction
- 00:53Sampling
- 01:46Fixation of the Samples
- 02:53Trimming the Fixed Sample
- 03:47Treatment with the Clearing Solution
- 04:46Results: Observation of the Depth of Fluorescence
- 06:32Conclusion
The present protocol describes a clearing technique for rice shoots, which are difficult to prepare for internal structural observations owing to the hard, thick, or layered nature of the tissues. This method facilitates continuous and deep fluorescence observations, even in adult rice plants.
