Journal
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生物学
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Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
JoVE 杂志
生物学
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JoVE 杂志 生物学
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

DOI:
10.3791/63990-v

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09:57 min

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October 17, 2022

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, , , , , , , ,
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology,The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology,The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology,The Fourth Military Medical University, 4School of Basic Medicine,The Fourth Military Medical University, 5College of Life Science,Northwest University, 6Xi’an Institute of Tissue Engineering and Regenerative Medicine

Chapters

  • 00:05Introduction
  • 01:20Preparation of the Plasma and the Maxillary Bone Samples and Extraction of Extracellular Vesicles
  • 02:50Origin Analysis of Extracellular Vesicles
  • 05:17Protein Content Analysis of Extracellular Vesicles
  • 08:06Results: Profiling of Surface Antigens and Protein Cargos of Extracellular Vesicles Isolated from In Vivo Samples
  • 09:13Conclusion

Summary

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The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos.

Tags

Keywords: Extracellular VesiclesIsolationAnalysisPlasmaSurface AntigensProtein CargoesFlow CytometryMaxillary BoneLiberaseCentrifugationPBSSample Processing

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