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Chemical Triphosphorylation of Oligonucleotides
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Chemical Triphosphorylation of Oligonucleotides

Chemical Triphosphorylation of Oligonucleotides

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13:19 min

June 02, 2022

DOI:

13:19 min
June 02, 2022

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Summary

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Oligonucleotide 5′-triphosphates are ubiquitous components in essential biological pathways and have seen increasing use in biotechnology applications. Here, we describe techniques for the routine synthesis and purification of oligonucleotide 5′-triphosphates, starting from oligonucleotides prepared by standard automated synthesis techniques.

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