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Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae

Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae

DOI:
10.3791/63874-v

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08:25 min

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April 21, 2022

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, ,
1Cluster of Excellence “Hearing4all” and Research Centre Neurosensory Science, Department of Neuroscience, School of Medicine and Health Science,Carl von Ossietzky University Oldenburg

Chapters

  • 00:05Introduction
  • 01:04Tissue Preparation
  • 02:21Fine Dissection of the Cochlea
  • 04:39Synapse Quantification
  • 06:52Results: Immunostaining of the Processed Cochleae
  • 07:50Conclusion

Summary

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A protocol for processing young adult and aged gerbil cochleae by immunolabeling the afferent synaptic structures and hair cells, quenching autofluorescence in aged tissue, dissecting and estimating the length of the cochleae, and quantifying the synapses in image stacks obtained with confocal imaging is presented.

Tags

Keywords: ImmunolabelingRibbon SynapsesGerbil CochleaAgingSynaptopathyCochlear DissectionAutofluorescence QuencherCochlear HalfRazor BladeSpring ScissorsForceps

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