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Chapters
- 00:04Introduction
- 01:17Mosquito Sampling, Sorting, and Grinding
- 02:15Preparation of Cells and Cell Culture Maintenance Medium
- 02:56Virus Isolation
- 04:38Results: Isolation and Amplification of Viruses Using Vertebrate and Mosquito Cell Lines
- 05:38Conclusion
Numerous novel virus-like sequences have been found in mosquitoes due to the extensive use of sequencing technologies. We provide an effective procedure for isolating and amplifying viruses using vertebrate and mosquito cell lines, which might serve as the basis for future studies on mosquito-associated viruses, including mosquito-borne and mosquito-specific viruses.
