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Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
JoVE 杂志
化学
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JoVE 杂志 化学
Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

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08:14 min

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December 09, 2022

DOI:

10.3791/63779-v

DOI:
10.3791/63779-v

•

08:14 min
December 09, 2022

•

8 Views
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1Hyphenated Mass Spectrometry Laboratory (HyMaS),University of Technology Sydney, 2School of Life Sciences,University of Technology Sydney

Summary

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The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.

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Non-protein Amino AcidsCyanobacteriaLiquid Chromatography-tandem Mass SpectrometryBMAAEG24-DABExtractionFreeze DryingSonicationCentrifugationTrichloroacetic Acid

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Cite this Article

Pravadali-Cekic, S., Violi, J. P., Mitrovic, S. M., Rodgers, K. J., Bishop, D. Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis. J. Vis. Exp. (190), e63779, doi:10.3791/63779 (2022).

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