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从人包皮中分离、培养和表征原代施旺细胞、角质形成细胞和成纤维细胞
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
DOI:

10:36 min

March 23, 2022

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Chapters

  • 00:04Introduction
  • 01:17Epidermis and Dermis Separation Procedure
  • 03:03Keratinocyte Isolation from the Epidermis
  • 05:02Isolation of Schwann Cells (SCs) and Fibroblast from the Dermis
  • 08:18Results: Characterization of Human Foreskin-Derived Primary Keratinocytes, Schwann Cells, and Fibroblasts
  • 09:43Conclusion

Summary

自动翻译

研究与肌肉骨骼损伤相关的伤口愈合通常需要评估施万细胞(SCs)、角质形成细胞和成纤维细胞之间的 体外 相互作用。该协议描述了从人包皮中分离,培养和表征这些原代细胞。

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