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Isolatie, kweek en karakterisering van primaire Schwann-cellen, keratinocyten en fibroblasten uit menselijke voorhuid
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神经科学
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JoVE 杂志 神经科学
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
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Isolatie, kweek en karakterisering van primaire Schwann-cellen, keratinocyten en fibroblasten uit menselijke voorhuid

4,916 Views

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10:36 min

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March 23, 2022

DOI:

10.3791/63776-v

DOI:
10.3791/63776-v

•

10:36 min
March 23, 2022

•

29 Views
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1Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science (CORTS),The Pennsylvania State University College of Medicine, 2Department of Dermatology,The Pennsylvania State University College of Medicine

Summary

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De studie van wondgenezing geassocieerd met musculoskeletale schade vereist vaak de beoordeling van in vitro interacties tussen Schwann-cellen (SC's), keratinocyten en fibroblasten. Dit protocol beschrijft de isolatie, kweek en karakterisering van deze primaire cellen uit de menselijke voorhuid.

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Cite this Article

Jagadeeshaprasad, M. G., Govindappa, P. K., Nelson, A. M., Elfar, J. C. Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin. J. Vis. Exp. (181), e63776, doi:10.3791/63776 (2022).

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