Journal
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生物化学
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Shotgun Lipidomics of Rodent Tissues
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Shotgun Lipidomics of Rodent Tissues

Shotgun Lipidomics of Rodent Tissues

DOI:
10.3791/63726-v

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11:46 min

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November 18, 2022

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, , ,
1PMI R&D,Philip Morris Products SA

Chapters

  • 00:04Introduction
  • 00:42Bradford Protein Determination Assay
  • 01:39BUME Extraction
  • 05:16MS Analysis
  • 09:31Results: Shotgun Lipidomics to Study the Effects of Cigarette Smoke on the Lungs of Mice
  • 11:01Conclusion

Summary

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Shotgun mass spectrometry-based lipidomics delivers a sensitive quantitative snapshot of a broad spectrum of lipid classes simultaneously in a single measurement from various rodent tissues.

Tags

LipidomicsLipid QuantificationBiomarkersToxicityLipid ClassesAmmonium BicarbonateBovine Serum AlbuminBradford ReagentInternal StandardsButanol-methanolHeptane-ethyl AcetateLiquid-liquid ExtractionMass Spectrometry

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