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Un test di pull-down a singola molecola ottimizzato per la quantificazione della fosforilazione delle proteine
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生物化学
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An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
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Un test di pull-down a singola molecola ottimizzato per la quantificazione della fosforilazione delle proteine

2,576 Views

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07:45 min

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June 06, 2022

DOI:

10.3791/63665-v

DOI:
10.3791/63665-v

•

07:45 min
June 06, 2022

•

6 Views
, , , , , , ,
1Department of Pathology,University of New Mexico School of Medicine, 2Department of Physics and Astronomy,University of New Mexico, 3Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Summary

Automatically generated

Il presente protocollo descrive la preparazione del campione e l'analisi dei dati per quantificare la fosforilazione proteica utilizzando un test di pull-down a singola molecola (SiMPull) migliorato.

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Cite this Article

Bailey, E. M., Salazar-Cavazos, E., Grattan, R. M., Wester, M. J., Schodt, D. J., Rojo, J. A., Lidke, K. A., Lidke, D. S. An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation. J. Vis. Exp. (184), e63665, doi:10.3791/63665 (2022).

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