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Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment
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发育生物学
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JoVE 杂志 发育生物学
Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

DOI:
10.3791/63651-v

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10:16 min

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March 28, 2022

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1Department of Biological and Vision Sciences, College of Optometry,The State University of New York

Chapters

  • 00:05Introduction
  • 00:48Eyeball Cleaning, Retina Extraction, Transfer, and Washing
  • 03:06Retina Dissociation
  • 05:20Cell Passage to Remove Neuronal Survivors
  • 07:12Results: Tracking of Mller Glia Conversion into Progenitor Cells and Neuronal Identity Validation
  • 09:24Conclusion

Summary

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Müller glia primary cultures obtained from mouse retinas represent a very robust and reliable tool to study the glial conversion into retinal progenitor cells after microRNA treatment. Single molecules or combinations can be tested before their subsequent application of in vivo approaches.

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