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Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications
 

Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications

Article DOI: 10.3791/63587-v • 08:46 min • April 21st, 2022
April 21st, 2022 •
1,2, 1, 2, 3, 1,2, 1
1Environmental and Occupational Health Sciences Institute, Rutgers University, 2Department of Biochemistry and Microbiology, Rutgers University, 3Centro de Química Estrutural and Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa

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The increased rate of pharmaco- and toxicokinetic analyses of metals and metal-based compounds in zebrafish can be advantageous for environmental and clinical translation studies. The limitation of unknown waterborne exposure uptake was overcome by conducting trace metal analysis on digested zebrafish tissue using inductively coupled plasma mass spectrometry.

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Platinum-based Compound Ruthenium-based Compound Exposure Zebrafish Inductively Coupled Plasma Mass Spectrometry ICPMS Waterborne Dosing Tissue Digestion Metals Quantification Dose Response Pharmacological Exposure Environmental Metal Exposure Nitric Acid Polypropylene Centrifuge Tubes Ultrasonication Tissue Oxidation Yellow Solution Dilution Calibration Curve Elemental Standard
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