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Gelişmekte Olan (Kurbağa) Embriyoda Hücre-Soy Kılavuzlu Kütle Spektrometrisi Proteomikleri
JoVE 杂志
化学
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JoVE 杂志 化学
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
DOI:

09:18 min

April 21, 2022

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Chapters

  • 00:04Introduction
  • 00:52Identification and Isolation of Single Cells
  • 01:27Labeling Cells of Interest
  • 02:42Isolation of Labeled Clones
  • 03:47Proteomic Analysis
  • 05:39Separation by Capillary Electrophoresis
  • 06:37Separation by Liquid Chromatography
  • 07:12Ionization and Detection of Peptides
  • 07:30Results: Characterization of Protein Expression in Single Cells and Cell Lineages of Xenopus laevis Embryos
  • 08:25Conclusion

Summary

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Burada, omurgalı Xenopus laevis embriyosunda bilinen doku kaderlerine sahip hücre soylarının kütle spektrometrisine dayalı proteomik karakterizasyonu açıklanmaktadır.

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