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Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
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JoVE 杂志 免疫与感染
Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

2,560 Views

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09:43 min

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June 08, 2022

DOI:

10.3791/63506-v

DOI:
10.3791/63506-v

•

09:43 min
June 08, 2022

•

7 Views
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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Summary

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The present protocol describes a method to track lupus progression in mice. Two additional procedures are presented to characterize lupus nephritis based on cell infiltration and protein deposition in the kidneys.

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ProteinuriaRenal InfiltrationLeukocytesRenal DepositionProteinsLupus-prone MRL/lpr MiceChronic InflammationKidneysLupusUrine CollectionKidney DissectionCell IsolationPercoll GradientLeukocyte PurificationFlow Cytometry

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Cabana-Puig, X., Luo, X. M. Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice. J. Vis. Exp. (184), e63506, doi:10.3791/63506 (2022).

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