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Primaire culturen van rat astrocyten en microglia en hun gebruik in de studie van amyotrofische laterale sclerose
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神经科学
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JoVE 杂志 神经科学
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
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Primaire culturen van rat astrocyten en microglia en hun gebruik in de studie van amyotrofische laterale sclerose

2,966 Views

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09:36 min

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June 23, 2022

DOI:

10.3791/63483-v

DOI:
10.3791/63483-v

•

09:36 min
June 23, 2022

•

17 Views
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1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”,University of Belgrade Faculty of Biology

Summary

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We presenteren hier een protocol over het voorbereiden van primaire culturen van gliacellen, astrocyten en microglia uit rattencorticosterces voor time-lapse videobeeldvorming van intracellulaire Ca2+ voor onderzoek naar pathofysiologie van amyotrofische laterale sclerose in het hSOD1G93A-rattenmodel .

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Cite this Article

Milićević, K., Korenić, A., Milošević, M., Andjus, P. R. Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis. J. Vis. Exp. (184), e63483, doi:10.3791/63483 (2022).

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