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大鼠星形胶质细胞和小胶质细胞的原代培养及其在肌萎缩侧索硬化症研究中的应用
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Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
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大鼠星形胶质细胞和小胶质细胞的原代培养及其在肌萎缩侧索硬化症研究中的应用

2,965 Views

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09:36 min

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June 23, 2022

DOI:

10.3791/63483-v

DOI:
10.3791/63483-v

•

09:36 min
June 23, 2022

•

17 Views
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1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”,University of Belgrade Faculty of Biology

Summary

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我们在这里提出了一个关于如何从大鼠皮质制备神经胶质细胞、星形胶质细胞和小胶质细胞的原代培养物的协议，用于细胞内 Ca2+ 的延时视频成像，用于研究 hSOD1G93A 大鼠模型中肌萎缩侧索硬化症的病理生理学。

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Cite this Article

Milićević, K., Korenić, A., Milošević, M., Andjus, P. R. Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis. J. Vis. Exp. (184), e63483, doi:10.3791/63483 (2022).

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