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Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
JoVE 杂志
生物学
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JoVE 杂志 生物学
Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

DOI:
10.3791/63445-v

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08:21 min

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May 16, 2022

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, ,
1Laboratory of Mycology, Department of Earth and Environmental Sciences,University of Pavia

Chapters

  • 00:04Introduction
  • 00:46Soil Sampling and Dilutions
  • 02:35Growth Tests on Recalcitrant Substances
  • 05:00Qualitative Enzymatic Tests
  • 06:26Results: Isolation and Screening of Fungal Strains for the Degradation of Recalcitrant Materials
  • 07:52Conclusion

Summary

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Here, we present a protocol for screening soil biodiversity to look for fungal strains involved in the degradation of recalcitrant materials. First, fungal strains able to grow on humic acids or lignocellulose are isolated. Their activity is then tested both in enzymatic assays and on pollutants such as hydrocarbons and plastics.

Tags

Keywords: Soil BiodiversityFungal DegradationRecalcitrant MaterialsBioremediationIsolationScreeningSelective MediaHumic AcidLignocelluloseFungal ColoniesMicroscopyMalt Extract AgarBushnell Haas Medium

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