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Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations

Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations

DOI:
10.3791/63416-v

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07:17 min

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October 28, 2022

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, , , , ,
1Department of Entomology and Nematology,University of Florida, 2Florida Museum of Natural History,University of Florida, 3Florida Natural Areas Inventory

Chapters

  • 00:04Introduction
  • 00:59Dissemination of Supplies to Community Scientists
  • 01:33Community Scientist Collection Preparation
  • 02:36Sample Collection in the Field
  • 05:37Results: A Field Method for Non-Destructively Sampling Rare Butterflies for Analyzing Population Genetic Structure
  • 06:36Conclusion

Summary

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自动翻译

Here, we present a straightforward protocol for noninvasive genetic sampling of butterfly populations based on the field collection of residual egg debris. It can be used to confirm species identity and quantify genetic variation. This protocol can be easily adapted to broader groups for community science involvement.

Tags

Genetic SamplingRare Butterfly PopulationsNondestructive SamplingChorionButterfly OviCommunity ScientistsLow CostEasy To Acquire SuppliesResidual Organic MaterialField CollectionMicrocentrifuge TubesLysis BufferUnique ID LabelsDigital EquipmentField StorageWeatherproof Container

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