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Ekstraksjon og rensing av FAHD1 Protein fra svin nyre og mus lever
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
DOI:

12:07 min

February 18, 2022

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Chapters

  • 00:04Introduction
  • 00:55Preparation of Buffer, Tissue Sample, and Solution
  • 02:15Total Protein Extraction
  • 03:42SDS-PAGE and Western Blot Analysis
  • 04:39Ammonium Sulfate Precipitation
  • 06:28Ionic Exchange Chromatography with FPLC
  • 08:14Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC
  • 09:32Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
  • 11:24Conclusion

Summary

自动翻译

Denne protokollen beskriver hvordan du trekker ut fumarylacetoaceaceacetathydrlase domeneholdig protein 1 (FAHD1) fra svin nyre og mus lever. De oppførte metodene kan tilpasses andre proteiner av interesse og modifiseres for andre vev.

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