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High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia
JoVE 杂志
医学
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JoVE 杂志 医学
High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia

High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia

DOI:
10.3791/63292-v

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05:32 min

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January 19, 2022

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, , ,
1Allergy Centre,Tampere University Hospital, 2Department of Pediatrics,Turku University Hospital, 3Turku Bioscience Center,University of Turku and Åbo Akademi University

Chapters

  • 00:04Introduction
  • 01:06Collection and Transport of Respiratory Epithelial Cells
  • 02:20High-Speed Video Microscopy Analysis (HSVMA)
  • 03:43Results: Analysis of Ciliary Motion in Healthy Individual and Primary Ciliary Dyskinesia (PCD) Patients
  • 04:49Conclusion

Summary

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High-speed video microscopy analysis is a relatively easy-to-perform, fast, cost-effective, and, in experienced hands, a considerably reliable tool for first-line diagnostics of primary ciliary dyskinesia, which should be available in every center involved in diagnostics and the treatment of severe lung diseases.

Tags

Keywords: High-speed Video MicroscopyPrimary Ciliary DyskinesiaFirst-line DiagnosisRespiratory Epithelial CellsCiliaDMEMMicroscopeOil Immersion LensVideo Recording

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