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Protocole basé sur la microdissection laser pour l’analyse LC-MS/MS du profil protéomique des granules de neuromélanine
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Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
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Protocole basé sur la microdissection laser pour l’analyse LC-MS/MS du profil protéomique des granules de neuromélanine

2,000 Views

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07:35 min

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December 16, 2021

DOI:

10.3791/63289-v

DOI:
10.3791/63289-v

•

07:35 min
December 16, 2021

•

32 Views
, , , , , , , , ,
1Medizinisches Proteom-Center, Medical Faculty,Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI),Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy,University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research,University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy,University Hospital of Wuerzburg, University of Wuerzburg

Summary

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Un protocole robuste est présenté ici pour isoler les granules de neuromélanine à partir de tissu humain post-mortem substantia nigra pars compacta par microdissection laser. Ce protocole révisé et optimisé minimise considérablement le temps requis pour le prélèvement des échantillons, réduit la quantité d’échantillon requise et améliore l’identification et la quantification des protéines par analyse LC-MS/MS.

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Cite this Article

Wulf, M., Barkovits-Boeddinghaus, K., Sommer, P., Schork, K., Eisenacher, M., Riederer, P., Gerlach, M., Kösters, S., Eggers, B., Marcus, K. Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules. J. Vis. Exp. (178), e63289, doi:10.3791/63289 (2021).

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