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Chemical Cartography Approaches to Study Trypanosomatid Infection
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January 21, 2022
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1Department of Chemistry and Biochemistry,University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research,University of Oklahoma, Norman, 3Department of Anthropology,University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology,University of Oklahoma, Norman
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This protocol describes the steps to generate a 3D model of metabolite distribution during trypanosomatid infection, including sample collection, metabolite extraction, an overview of liquid chromatography-tandem mass spectrometry data acquisition, 3D model generation, and finally, data visualization.
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Dean, D. A., Haffner, J. J., Katemauswa, M., McCall, L. Chemical Cartography Approaches to Study Trypanosomatid Infection. J. Vis. Exp. (179), e63255, doi:10.3791/63255 (2022).
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