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Aislamiento de células endoteliales pulmonares primarias de ratón
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Isolation of Primary Mouse Lung Endothelial Cells
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Aislamiento de células endoteliales pulmonares primarias de ratón

3,702 Views

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06:41 min

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November 10, 2021

DOI:

10.3791/63253-v

DOI:
10.3791/63253-v

•

06:41 min
November 10, 2021

•

15 Views
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1Department of Pediatrics, Division of Critical Care,UCSF Benioff Children’s Hospital, 2Department of Anesthesia and Perioperative Care,University of California, San Francisco

Tags

Primary Mouse LungEndothelial CellsIsolationCell CultureCD31CD102ImmunobeadsCollagenaseHBSSDMEMCell StrainerTrituration

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Wong, E., Nguyen, N., Hellman, J. Isolation of Primary Mouse Lung Endothelial Cells. J. Vis. Exp. (177), e63253, doi:10.3791/63253 (2021).

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