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Isolamento de Células Endoteliais Primárias de Pulmão de Camundongo

JoVE 杂志
免疫与感染

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JoVE 杂志免疫与感染

Isolation of Primary Mouse Lung Endothelial Cells

Isolamento de Células Endoteliais Primárias de Pulmão de Camundongo

3,702 Views

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06:41 min

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November 10, 2021

DOI:

10.3791/63253-v

DOI:

10.3791/63253-v

•

06:41 min

November 10, 2021

•

15 Views

Erika Wong², Nina Nguyen, Judith Hellman

¹Department of Pediatrics, Division of Critical Care,UCSF Benioff Children’s Hospital, ²Department of Anesthesia and Perioperative Care,University of California, San Francisco

Tags

Primary Mouse Lung Endothelial Cells Isolation Cell Culture CD31 CD102 Immunobeads Collagenase HBSS DMEM Cell Strainer Trituration

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