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Generering af nullmutanter for at belyse rollen som bakterielle glycosyltransferaser i bakteriel motilitet
Chapters
- 00:04Introduction
- 01:01Construction of pDM4 Recombinant Plasmid with PCR In-Frame Deletion Products
- 03:10Preparation of Electrocompetent E. coli and Electroporation of pDM4 Recombinant Plasmid
- 04:52Transfer of In-Frame Deletion to the Aeromonas Strain
- 07:44Motility Assays in Liquid and Semi-Solid Media
- 08:34Isolation and Purification of Flagella
- 10:23Results: Confirmation of Null Mutants and Phenotypic Analysis of Polar Flagella
- 11:47Conclusion
Her beskriver vi konstruktionen af nulmutanter af Aeromonas i specifikke glycosyltransferaser eller regioner indeholdende glycosyltransferaser, motilitetsassays og flagellarensning udført for at fastslå involvering og funktion af deres kodede enzymer i biosyntesen af en glycan samt denne glycans rolle i bakteriel patogenese.
