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Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma
JoVE 杂志
医学
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JoVE 杂志 医学
Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

DOI:
10.3791/63083-v

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07:53 min

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April 15, 2022

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, , , , , , , , ,
1Department of Hematology,Zhongnan Hospital of Wuhan University, 2Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology

Chapters

  • 00:09Introduction
  • 01:23Preparation of Bone Marrow Smears
  • 02:37FISH Pre-Processing of Bone Marrow Smear
  • 03:48Bone Marrow Smear FISH and Washing
  • 05:20FISH Analysis and Imaging
  • 06:20Representative Result
  • 07:22Conclusion

Summary

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Here, we present a protocol for improving the success of interphase fluorescence in situ hybridization detection on bone marrow smears from multiple myeloma patients.

Tags

Keywords: InterphaseFluorescence In Situ HybridizationBone Marrow SmearsMultiple MyelomaPlasma CellsCIg FISHGenetic Risk StratificationWright-Giemsa StainingMicroscopyFixative SolutionSSC Buffer

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