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In Vivo Wireless Optogenetic Control of Skilled Motor Behavior
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神经科学
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JoVE 杂志 神经科学
In Vivo Wireless Optogenetic Control of Skilled Motor Behavior

In Vivo Wireless Optogenetic Control of Skilled Motor Behavior

DOI:
10.3791/63082-v

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07:52 min

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November 22, 2021

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, , , , ,
1Institute of Cellular Physiology,National University of Mexico, 2Department of Neurosurgery,Stanford University, 3Facultad de Psicologia,National University of Mexico, 4Brain Mechanisms for Behaviour Unit,Okinawa Institute of Science and Technology Graduate University

Chapters

  • 00:04Introduction
  • 00:57Surgical Procedures
  • 04:48Reach-To-Grasp Training
  • 05:25Results: Kinematic Analysis of Reaching Behavior and Optogenetic Activation of Contralateral and Ipsilateral D1 Spiny Projection Neurons (SPNs)
  • 07:10Conclusion

Summary

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The present protocol describes how to use wireless optogenetics combined with high-speed videography in a single pellet reach-to-grasp task to characterize the neural circuits involved in the performance of skilled motor behavior in freely moving mice.

Tags

Wireless OptogeneticsSkilled Motor BehaviorHigh-speed VideographyNeurodevelopmental DisordersNeurodegenerative DisordersLED CannulaMicroinjectionsStereotaxic ApparatusBregmaLambda

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