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JoVE Journal Developmental Biology

在自制迷你生物反应器中从诱导多能干细胞中生成脑类器官

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在自制迷你生物反应器中从诱导多能干细胞中生成脑类器官

Article DOI: 10.3791/62987-v • 10:16 min • December 11th, 2021

December 11th, 2021 •

Artem Eremeev^1,3, Lilia Belikova^1,2, Evgeny Ruchko¹, Egor Volovikov^1,5, Olga Zubkova¹, Alexy Emelin⁴, Roman Deev⁴, Olga Lebedeva^1,3, Alexandra Bogomazova^1,3, Maria Lagarkova^1,3

¹Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, ²Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, ³Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, ⁴Department of Pathological Anatomy, North-Western State Medical University, ⁵Department of Biology, Lomonosov State University

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在这里，我们描述了一种从人类诱导多能干细胞（iPSCs）产生脑类器官的方案。为了获得大量和高质量的脑类器官，我们使用自制的迷你生物反应器。

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《发育生物学》第178期，

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