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Immunology and Infection

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JoVE Journal Immunology and Infection
Plaquing van Herpes Simplex Virussen
 
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Plaquing van Herpes Simplex Virussen

Article DOI: 10.3791/62962-v • 04:41 min • November 5th, 2021
November 5th, 2021 •
*1, *1, *1, *1,2, 3, 1,4
1Towson University Herpes Virus Lab, Department of Biological Sciences, Towson University, 2Towson University Department of Chemistry, Towson University, 3Department of Oncology, Johns Hopkins University School of Medicine, 4Molecular Biology, Biochemistry, and Bioinformatics Program, Towson University
* These authors contributed equally

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Plaquing is een routinemethode die wordt gebruikt om levende virussen in een populatie te kwantificeren. Hoewel plaquing vaak wordt onderwezen in verschillende microbiologische curricula met bacteriën en bacteriofagen, is het plaquenten van zoogdiervirussen complexer en tijdrovender. Dit protocol beschrijft de procedures die betrouwbaar functioneren voor regelmatig werk met herpes simplex-virussen.

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Immunologie en infectie nummer 177
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