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Chapters
- 00:05Introduction
- 00:24Prepare Assay Solutions for the Screen
- 01:48Prepare Enzyme Solutions for the Screen
- 02:52Run the Methylation Assay and Analyze the Data
- 04:29Results: Determination of Initial Velocities of DNA Methylation in the Presence and Absence of Potential Inhibitors
- 05:40Conclusion
DNA-metyltransferaser er potensielle kreftmedisinmål. Her presenteres en protokoll for å vurdere små molekyler for DNA-metyltransferasehemming. Denne analysen benytter en endonuklease for å koble DNA-metylering til fluorescensgenerering og gjør det mulig å overvåke enzymaktivitet i sanntid.
