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Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples
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神经科学
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JoVE 杂志 神经科学
Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples

Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples

3,766 Views

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08:45 min

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August 04, 2021

DOI:

10.3791/62914-v

DOI:
10.3791/62914-v

•

08:45 min
August 04, 2021

•

20 Views
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1Neurobiology, Neurodegeneration & Repair Laboratory, National Eye Institute,National Institutes of Health

Summary

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Described here is a detailed protocol for performing mitochondrial stress assay and glycolytic rate assay in ex vivo retinal tissue samples using a commercial bioanalyzer.

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Cite this Article

Jiang, K., Nellissery, J., Swaroop, A. Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples. J. Vis. Exp. (174), e62914, doi:10.3791/62914 (2021).

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