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发育生物学
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Direct Reprogramming of Mouse Fibroblasts into Melanocytes
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发育生物学
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JoVE 杂志 发育生物学
Direct Reprogramming of Mouse Fibroblasts into Melanocytes

Direct Reprogramming of Mouse Fibroblasts into Melanocytes

DOI:
10.3791/62911-v

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09:38 min

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August 27, 2021

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, , , , ,
1Institute for Regenerative Medicine,Jiangsu University, 2Department of Dermatology,Affiliated Hospital of Jiangsu University

Chapters

  • 00:04Introduction
  • 01:21Production of the Concentrated Lentivirus
  • 03:22Detection of Concentrated Virus Titer
  • 04:39Direct Reprogramming of Fibroblasts to Melanocytes
  • 07:17Results: Direct Reprogramming of Mouse Embryonic Fibroblasts (MEF) into Melanocytes Using Transcription Factors
  • 08:46Conclusion

Summary

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Here, we describe an optimized direct reprogramming system for melanocytes and a high-efficiency, concentrated virus packaging system that ensures smooth direct reprogramming.

Tags

Direct ReprogrammingMelanocytesTranscription FactorsMITFSOX10PAX3Lentivirus ProductionHEK-293T CellsVirus Concentration

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