Journal
/
生物学
/
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
JoVE 杂志
生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

DOI:
10.3791/62845-v

•

09:36 min

•

August 26, 2021

•

, ,
1Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill School of Medicine

Chapters

  • 00:04Introduction
  • 00:56Fixation and Preparation
  • 02:21Direct Stochastic Optical Reconstruction Microscopy Calibration
  • 04:56Visualization of EV in Three Dimensions
  • 06:16Post-Acquisition Modification and EV Tracing
  • 07:13Results: Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles
  • 09:00Conclusion

Summary

自动翻译

自动翻译

Direct stochastic optical reconstruction microscopy (dSTORM) is used to bypass the typical diffraction limit of light microscopy and to view exosomes at the nanometer scale. It can be employed in both two and three dimensions to characterize exosomes.

Tags

Keywords: Extracellular VesiclesDSTORMSuper-resolution MicroscopyThree-dimensional VisualizationVirus ParticlesDisease BiomarkersAffinity PurificationParaformaldehyde FixationImaging BufferCalibration Beads

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/62845?article_type=v

Phase Contrast and Differential Interference Contrast (DIC) Microscopy
check_url/cn/62845?article_type=v

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
check_url/cn/62845?article_type=v

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory
check_url/cn/62845?article_type=v

Hemi-laryngeal Setup for Studying Vocal Fold Vibration in Three Dimensions
check_url/cn/62845?article_type=v

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
check_url/cn/62845?article_type=v

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
check_url/cn/62845?article_type=v

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
check_url/cn/62845?article_type=v

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
check_url/cn/62845?article_type=v

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy
check_url/cn/62845?article_type=v

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Read Article