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Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

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Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

DOI:

10.3791/62820-v

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06:28 min

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September 24, 2021

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Yen Xuan Ngo^2,3, Kenta Haga, Ayako Suzuki, Hiroko Kato, Hiromi Yanagisawa⁵, Kenji Izumi, Aiko Sada³

¹Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ²Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba, ³International Research Center for Medical Sciences (IRCMS),Kumamoto University, ⁴Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences,Niigata University, ⁵Faculty of Medicine,University of Tsukuba

Summary

自动翻译

The present protocol describes the isolation and culture of oral keratinocytes derived from the adult mouse palate. An evaluation method using immunostaining is also reported.

Tags

Primary Oral Keratinocytes Mouse Palate Stem Cell-like State Long-term Culture Epithelial Layer Trypsin Digestion Lamina Propria Cell Isolation Culture Technique

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