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生物工程
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Rapid Characterization of Genetic Parts with Cell-Free Systems
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Rapid Characterization of Genetic Parts with Cell-Free Systems

Rapid Characterization of Genetic Parts with Cell-Free Systems

DOI:
10.3791/62816-v

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05:00 min

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August 30, 2021

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, , , , , , ,
1US Army Combat Capabilities Development Command Army Research Laboratory, 2Biology and Biological Engineering,California Institute of Technology, 3Excet, Inc., 4US Army Combat Capabilities Development Command Chemical Biological Center

Chapters

  • 00:04Introduction
  • 00:39Linear Template Preparation
  • 02:11Cell-Free Protein Synthesis
  • 03:44Results: Rapid Characterization of Genetic Parts
  • 04:34Conclusion

Summary

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Well-characterized genetic parts are necessary for the design of novel genetic circuits. Here we describe a cost-effective, high-throughput method for rapidly characterizing genetic parts. Our method reduces cost and time by combining cell-free lysates, linear DNA to avoid cloning, and acoustic liquid handling to increase throughput and reduce reaction volumes.

Tags

Keywords: Cell-free Expression SystemsGenetic Part CharacterizationPCRRapid PrototypingDNA Template PurificationMaster Mix PreparationCell-free Expression Setup

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