Embryonic development requires large-scale coordination of cell motion. Two-photon excitation mediated laser ablation allows the spatially controlled 3-dimensional ablation of large groups of deep cells. In addition, this technique can probe the reaction of collectively migrating cells in vivo to perturbations in their mechanical environment.
Boutillon, A., Escot, S., David, N. B. Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula. J. Vis. Exp. (173), e62815, doi:10.3791/62815 (2021).