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测量来自母猪后代的先前冷冻心脏组织中的线粒体电子转移复合物：评估运动诱导的线粒体生物能量变化的模型
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08:04 min
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August 16, 2021
DOI:
1AdventHelath, 2University of Illinois Chicago School of Public Health, 3Lincoln Memorial University, 4Children’s Mercy Hospital, 5Scripps Clinic, 6Cincinnati Children’s Hospital Medical Center, 7Kansas City University, 8Roblex Rex Veterans Affairs Medical Center, 9California State University San Marcos, 10East Carolina University, 11Massachusetts Institute of Technology, 12Boehringer Ingelheim Norway KS, 13Heartland Center for Mitochondrial Medicine
Summary
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从先前冷冻的存档固体组织中制备富含线粒体的样品，使研究人员能够以各种实验方式对线粒体进行功能和分析评估。这项研究展示了如何从冷冻的心脏组织制备富含线粒体的制剂，并对线粒体进行分析评估。
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Cite this Article
Barrera, D., Upton, S., Rauch, M., Notarianni, T., Eum, K. S., Liberty, M., Sah, S. V., Liu, R., Newcomer, S., May, L. E., Agbas, E., Sage, J., Kosa, E., Agbas, A. Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes. J. Vis. Exp. (174), e62809, doi:10.3791/62809 (2021).
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