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Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
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Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants

Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants

DOI:
10.3791/62781-v

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04:16 min

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August 05, 2021

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, , , , ,
1Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS),University of the Balearic Islands, 2Health Research Institute of the Balearic Islands (IdISBa), 3Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB), 4Department of Fundamental Biology and Health Sciences,University of the Balearic Islands

Chapters

  • 00:04Introduction
  • 00:40Titanium Discs Wash
  • 01:43Titinium Passivation and Extracellular Vesicles (EVs) Drop Casting
  • 03:04Results: Accumulative EV Release of Titanium Functionalized Surfaces and In Vitro Cell Biocompatibility of Titanium-EVs
  • 03:50Conclusion

Summary

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Here, we present a method for the isolation of Extracellular Vesicles (EVs) derived from the platelet lysates (PL) and their use for coating titanium (Ti) implant surfaces. We describe the drop casting coating method, the EVs release profile from the surfaces, and in vitro biocompatibility of EVs coated Ti surfaces.

Tags

Keywords: Titanium ImplantsExtracellular VesiclesSurface FunctionalizationDrop CastingBone RegenerationNanoparticle Tracking AnalysisSodium HydroxideNitric AcidVacuum Drying

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