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Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
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Chapters
- 00:03Introduction
- 01:15Determination (in silico) of the ANS and SERCA N‐Domain Interaction
- 03:09Expression and Purification of the Recombinant N‐Domain
- 03:39Monitor the Formation of the ANS‐N‐Domain Complex Based on ANS and N‐Domain Fluorescence Intensity Changes
- 07:38N‐Domain Intrinsic Fluorescence Titration by Trp Chemical Modification with NBS
- 08:43Titrate the NBS Modified N‐Domain with ANS by Recording Fluorescence Spectra at 25°C
- 09:37Evidence of ANS Binding to the Chemically Modified N‐Domain by Excitation at λ = 370 nm
- 10:32Results Overview
- 11:30Conclusions
ANS binds to the Ca2+-ATPase recombinant N-domain. Fluorescence spectra display a FRET-like pattern upon excitation at a wavelength of 295 nm. NBS-mediated chemical modification of Trp quenches the fluorescence of the N-domain, which leads to the absence of energy transfer (FRET) between the Trp residue and ANS.
