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Automated, High-Throughput Detection of Bacterial Adherence to Host Cells
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JoVE 杂志 免疫与感染
Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

DOI:
10.3791/62764-v

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07:21 min

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September 17, 2021

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, , , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2College of Veterinary Medicine,Texas A&M University

Chapters

  • 00:04Introduction
  • 00:48Staining
  • 02:20Bacterial Adherence Assay
  • 04:01Automated Fluorescence Imaging, Processing, and Analysis
  • 05:24Results: Analysis of Host Cell-Adhered Bacteria
  • 06:46Conclusion

Summary

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Detection of host-bacterial pathogen interactions based on phenotypic adherence using high-throughput fluorescence labeling imaging along with automated statistical analysis methods enables rapid evaluation of potential bacterial interactions with host cells.

Tags

Bacterial AdherenceHigh-throughput DetectionHost CellsAutomatedTime-savingReproducibleMultiplicity Of InfectionBacterial SuspensionStainingBacterial Adherence AssayA549 CellsPBSFormaldehyde

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