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Optimizing the Setup and Conditions for Ex Vivo Electroretinogram to Study Retina Function in Small and Large Eyes
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神经科学
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JoVE 杂志 神经科学
Optimizing the Setup and Conditions for Ex Vivo Electroretinogram to Study Retina Function in Small and Large Eyes

Optimizing the Setup and Conditions for Ex Vivo Electroretinogram to Study Retina Function in Small and Large Eyes

DOI:
10.3791/62763-v

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06:41 min

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June 27, 2022

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, ,
1John A. Moran Eye Center,University of Utah

Chapters

  • 00:04Introduction
  • 00:47Setting up Ex Vivo ERG
  • 02:58Tissue Preparation and Mounting
  • 04:44Results: Ex Vivo Photoreceptor and ON‐Bipolar Cell Responses by ERG
  • 06:02Conclusion

Summary

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Modification of existing multielectrode array or patch clamp equipment makes the ex vivo electroretinogram more widely accessible. Improved methods to record and maintain ex vivo light responses facilitate the study of photoreceptor and ON-bipolar cell function in the healthy retina, animal models of eye diseases, and human donor retinas.

Tags

Ex Vivo ElectroretinogramRetina FunctionMulti-electrode ArrayDifferential AmplifierLED Light StimuliSilver Silver Chloride ElectrodeLarge EyesSmall EyesPharmacological AgentsRetinal NeuronsON-bipolar CellsSignal To Noise RatioRetinal Function QuantificationRetinal Disease

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