Journal
/
免疫与感染
/
Estudio del desarrollo de células dendríticas mediante la eliminación de genes mediados por ARN de horquilla corta en una línea de células madre y progenitoras hematopoyéticas in vitro
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
Please note that all translations are automatically generated. Click here for the English version.

Estudio del desarrollo de células dendríticas mediante la eliminación de genes mediados por ARN de horquilla corta en una línea de células madre y progenitoras hematopoyéticas in vitro

3,258 Views

•

06:12 min

•

March 07, 2022

DOI:

10.3791/62730-v

DOI:
10.3791/62730-v

•

06:12 min
March 07, 2022

•

2 Views
, ,
1Graduate Institute of Immunology,National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology,University of Utah

Tags

Dendritic Cell DevelopmentShort Hairpin RNAGene KnockdownHematopoietic Stem And Progenitor Cell LineIn VitroTranscription FactorsFLT3 LigandBeta EstradiolLentiviral TransductionPuromycinLacZTcf4Id2Flow Cytometry

Read Article

Cite this Article

Hsiao, Y., Häcker, H., Lee, C. Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro. J. Vis. Exp. (181), e62730, doi:10.3791/62730 (2022).

Download .ris file

Copy

Share Video

.

Copy