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Studio dello sviluppo delle cellule dendritiche mediante knockdown genico mediato da RNA a forcina corta in una linea cellulare ematopoietica e progenitrice in vitro
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JoVE 杂志 免疫与感染
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
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Studio dello sviluppo delle cellule dendritiche mediante knockdown genico mediato da RNA a forcina corta in una linea cellulare ematopoietica e progenitrice in vitro

3,258 Views

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06:12 min

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March 07, 2022

DOI:

10.3791/62730-v

DOI:
10.3791/62730-v

•

06:12 min
March 07, 2022

•

2 Views
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1Graduate Institute of Immunology,National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology,University of Utah

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Dendritic Cell DevelopmentShort Hairpin RNAGene KnockdownHematopoietic Stem And Progenitor Cell LineIn VitroTranscription FactorsFLT3 LigandBeta EstradiolLentiviral TransductionPuromycinLacZTcf4Id2Flow Cytometry

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Hsiao, Y., Häcker, H., Lee, C. Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro. J. Vis. Exp. (181), e62730, doi:10.3791/62730 (2022).

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