Journal
/
免疫与感染
/
Crecimiento, Purificación, Y La Titulación Del Virus Del Herpes Simple Oncolítico
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
Please note that all translations are automatically generated. Click here for the English version.

Crecimiento, Purificación, Y La Titulación Del Virus Del Herpes Simple Oncolítico

5,977 Views

•

06:14 min

•

May 13, 2021

DOI:

10.3791/62677-v

DOI:
10.3791/62677-v

•

06:14 min
May 13, 2021

•

21 Views
, , , ,
1Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy,Texas Tech University Health Sciences Center, 2Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery,Massachusetts General Hospital and Harvard Medical School

Summary

Automatically generated

En este manuscrito, describimos un método simple de crecimiento, de purificación, y de titulación del virus oncolítico del simplex de herpes para el uso preclínico.

Explore More Videos

Oncolytic Herpes Simplex VirusCancer ImmunotherapyVirus PropagationVirus PurificationVirus TitrationBiosafety Level TwoVirus StockAnticancer Immune ResponseVirus-based Cancer ImmunotherapyWild-type Herpes Simplex VirusGenetically Engineered Herpes Simplex VirusT-150 Square Centimeter FlasksDPBSIFCSDMEMCell ScraperCentrifugationVirus BufferBenzonase NucleaseVirus Titer

Read Article

Cite this Article

Nguyen, H., Sah, N., Humphrey, M. R. M., Rabkin, S. D., Saha, D. Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus. J. Vis. Exp. (171), e62677, doi:10.3791/62677 (2021).

Download .ris file

Copy

Share Video

.

Copy