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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

DOI:
10.3791/62663-v

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13:53 min

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August 26, 2021

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, ,
1Department of Chemical Pathology, Faculty of Health Sciences,University of Pretoria, 2Tshwane Academic Division,National Health Laboratory Service

Chapters

  • 00:04Introduction
  • 00:43Preparation of Cell Culture and Treatments for Flow Cytometry
  • 02:56Enumerating Cells Using the Viability Assay
  • 03:36Staining Cells for Flow Cytometry
  • 08:00Run Samples on the Flow Cytometer
  • 10:04Post-Acquisition and Statistical Analysis
  • 11:20Results: Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
  • 13:01Conclusion

Summary

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自动翻译

Apoptosis can be characterized by flow cytometric analysis of early and late apoptotic biomarkers. The cervical cancer cell line, SiHa, was analyzed for apoptosis biomarkers after treatment with Actinomycin D using a benchtop flow cytometer.

Tags

Keywords: Flow CytometryApoptosisActinomycin DSiHa Cervical Cancer CellsBiomarkersAnnexin VCell CultureTrypsinPhosphatidylserineDNA Binding Dyes

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