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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

3,137 Views

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13:53 min

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August 26, 2021

DOI:

10.3791/62663-v

DOI:
10.3791/62663-v

•

13:53 min
August 26, 2021

•

10 Views
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1Department of Chemical Pathology, Faculty of Health Sciences,University of Pretoria, 2Tshwane Academic Division,National Health Laboratory Service

Summary

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Apoptosis can be characterized by flow cytometric analysis of early and late apoptotic biomarkers. The cervical cancer cell line, SiHa, was analyzed for apoptosis biomarkers after treatment with Actinomycin D using a benchtop flow cytometer.

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Punchoo, R., Zhou, E., Bhoora, S. Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells. J. Vis. Exp. (174), e62663, doi:10.3791/62663 (2021).

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