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En effektiv metode for rettet hepatocyttlignende celleinduksjon fra humane embryonale stamceller
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JoVE 杂志 免疫与感染
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
DOI:

08:05 min

May 06, 2021

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Chapters

  • 00:04Introduction
  • 00:45Stem Cell Maintenance
  • 02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
  • 03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
  • 05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
  • 07:14Conclusion

Summary

自动翻译

Dette manuskriptet beskriver en detaljert protokoll for differensiering av humane embryonale stamceller (hESCer) i funksjonelle hepatocyttlignende celler (HLCer) ved kontinuerlig å supplere Activin A og CHIR99021 under hESC-differensiering til definitiv endoderm (DE).

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