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Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice
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神经科学
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JoVE 杂志 神经科学
Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice

Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice

DOI:
10.3791/62611-v

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06:23 min

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June 05, 2021

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, , ,
1Department of Veterinary Medicine, School of Veterinary Medicine,National Taiwan University

Chapters

  • 00:05Introduction
  • 00:42Adeno-Associated Virus (AAV) Injection and Relay Lens Implantation
  • 01:45Dummy Baseplating
  • 02:44Baseplating
  • 04:39Results: Assessment of Dental Cement and Monitoring Baseplating Procedure
  • 05:45Conclusion

Summary

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The shrinkage of dental cement during curing displaces the baseplate. This protocol minimizes the problem by creating an initial foundation of the dental cement that leaves space to cement the baseplate. Weeks later, the baseplate can be cemented in position on this scaffold using little new cement, therebyreducing shrinkage.

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BaseplatingMiniscopePreanchoredObjective LensCalcium TransientMiceFluorescence SignalsViral VectorRelay LensDental CementStereotaxic ArmMicroinjectionVentral Cornu Ammonis 1Pyrogen-free SalineMicro Bulldog ClampHeat Shrink TubingParaffin FilmReusable Adhesive ClayMolding Silicone RubberBone Rongeur75% AlcoholSet ScrewFocus Slide

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